ICAM1 antibody (Biotin)

Catalog No. ABIN111935

The Mouse Monoclonal anti-ICAM1 antibody has been validated for WB, FACS, IHC (p) and IHC (fro). It is suitable to detect ICAM1 in samples from Human.

Quick Overview for ICAM1 antibody (Biotin) (ABIN111935)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1304

Product Details anti-ICAM1 Antibody (Biotin)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein A Chromatography.

Immunogen: Cultured Human endothelial cells

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 0.25 μg/mL (1/800). Immunohistochemistry on Paraffin Sections: 2 μg/mL (1/100). Proteinase K pretreatmentfor antigen retrieval is recommended. Suggested Positive Control: Human tonsil. Has been described to work in FACS and Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: Stock Solution contains PBS, pH 7.2 with 0.01 % Thimerosal as preservative and 1 % BSA as stabilizer.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody undiluted Prior to and following reconstitution at 2-8 °C. DO NOT FREEZE.

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: CD54 / ICAM1

Background: ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.Synonyms: ICAM-1, Intercellular adhesion molecule 1, Major Group Rhinovirus Receptor

Gene ID: 9606

UniProt: P05362

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling